Ready-to-use media
Peptone Sorbitol Bile Broth (PBS) ISO
Enrichment of Yersinia sp. from food samples, formulated according to ISO 10273.
- Cat. No.
- LC-4683
- Category
- Ready-to-use media
- Format
- 10 x 100 mL flasks
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 6 months
- Minimum order
- 5 packs
- Standards
- ISO 10273
Medium for the pre-enrichment of food samples contaminated with presumptive Yersinia enterocolitica.
Key features
Applications
Selective enrichment of Yersinia enterocolitica.
Industry
Food.
Standards
ISO 10273.
Presentation
10 prepared bottles, 125 mL, with 100 ± 3 mL.
Description and technique
Technique
Use the medium according to ISO regulations.
Presentation
| Presentation | 10 prepared bottles, 125 mL |
|---|---|
| Filling | 100 ± 3 mL |
| Packaging | 1 box with 10 bottles 125 mL. Non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| Casein peptone | 5.00 |
| Sodium chloride | 5.00 |
| Sorbitol | 10.00 |
| Disodium phosphate 12 H₂O | 8.23 |
| Sodium phosphate 1 H₂O | 1.20 |
| Bile salts | 1.50 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.6 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes - inoculate with 100 ± 20 CFU for growth promotion or 10⁴-10⁶ CFU for selectivity.
Subculture in appropriate culture media (CIN Agar).
Aerobiosis. Incubation at 25 ± 1 °C, reading at 24-48-72 h to 5 days.
| Microorganism | Growth |
|---|---|
| Y. enterocoliticaATCC 9610 + ATCC 8739 + ATCC 27853 | > 10 CFU. Characteristic colonies of Yersinia in CIN Agar |
| Y. enterocoliticaATCC 23715 + ATCC 8739 + ATCC 27853 | > 10 CFU. Characteristic colonies of Yersinia in CIN Agar |
| Ps. aeruginosaATCC 27853, WDCM 00025 | Partial inhibition |
| Proteus mirabilisATCC 29906, WDCM 00023 | Partial inhibition |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ISO 10273 Standard (2003) Microbiology of food and animal feeding stuffs - Horizontal methods for the detection of presumptive pathogenic Yersinia enterocolitica.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
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