Ready-to-use media
Preston Broth without Blood
Selective liquid broth for isolation of Campylobacter spp.
- Cat. No.
- LC-4676
- Category
- Ready-to-use media
- Format
- 10 x 90 mL flasks
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 2 months
- Minimum order
- 10 packs
This medium supplemented with blood, antibiotics and reducing agents is used as a selective enrichment broth in the detection and isolation of Campylobacter from very contaminated samples.
Key features
Applications
Selective enrichment of Campylobacter.
Industry
Food.
Presentation
10 prepared bottles, 125 mL, with 90 ± 3 mL.
Packaging
1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended.
Description and technique
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
To obtain Preston Broth for enrichment of Campylobacter, add lysed blood in a proportion of 5-7% (v/v).
Inoculate the bottles aseptically with the prepared sample or its dilution. Incubate the bottles in microaerophilic atmosphere at 41.5 ± 1 °C for 44 ± 4 h.
Subculture onto appropriate secondary selective media for better isolation.
Presumptive isolation and recovery of Campylobacter must be confirmed by further microbiological and biochemical tests.
Each laboratory must evaluate the results according to their specifications.
Presentation
| Presentation | 10 prepared bottles, 125 mL |
|---|---|
| Filling | 90 ± 3 mL |
| Packaging | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Casein peptone | 10.00 |
| Meat peptone | 10.00 |
| Sodium chloride | 5.00 |
| Agar | 1.00 |
| Cycloheximide | 0.10 |
| Trimethoprim | 0.01 |
| Rifampicin | 0.01 |
| Polymyxin B | 5000 IU |
Add immediately before use: lysed horse blood, 5-7%.
Quality control
Physical/chemical control
| Color | Brown |
|---|---|
| pH | 7.4 ± 0.2 at 25 °C |
Microbiological control
Prior addition of 5% defibrinated sheep blood.
Prepare tubes - inoculate with 100 ± 20 CFU for growth promotion or 10⁴-10⁶ CFU for selectivity.
Microaerophilia. Incubation at 41.5 ± 1 °C; reading at 44 ± 4 h.
| Microorganism | Growth |
|---|---|
| Camp. coliATCC 43478, WDCM 00004 | Good |
| Campylobacter jejuniATCC 29428, WDCM 00156 | Good |
| Escherichia coliATCC 8739, WDCM 00012 | Partial inhibition |
| Stph. aureusATCC 25923, WDCM 00034 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- BOLTON, F.J. & L. ROBERTSON (1982) A selective medium for isolating Campylobacter jejuni/coli. J. Clin. Pathol. 35:462-467.
- BOLTON, F.J., D. COATES, P.M. HINCHLIFFE & L. ROBERTSON (1983) Comparative of selective media for isolation of Campylobacter jejuni/coli. J. Clin. Pathol. 36:78-83.
- CORRY, J.E.L., H.I. ATABAY, S.J. FORSYTHE & L.P. MANSFIELD (2003) Culture Media for the Isolation of Campylobacters, Helicobacters and Arcobacters, in Corry et al. (Eds) Handbook of Culture Media for Food Microbiology Chap 18 pgs 271-316. Elsevier Science B.V. Amsterdam.
- ISO 10272-1 Standard (2017) Microbiology of the food chain - Horizontal Method for detection and enumeration of Campylobacter spp. - Part 1: Detection method.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
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