Ready-to-use media
Malt Extract Agar with Chloramphenicol
Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples.
- Cat. No.
- LC-4674
- Category
- Ready-to-use media
- Format
- 10 x 200 mL flasks
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 6 months
- Minimum order
- 10 packs
Malt Extract Agar promotes the growth of almost all fungi because of its balanced composition, and its ability to inhibit bacteria due to its low pH and the presence of chloramphenicol.
Key features
Applications
Selective enumeration and selective isolation of yeasts and molds.
Industry
Water.
Presentation
10 prepared bottles, 250 mL, with 200 ± 5 mL.
Packaging
1 box with 10 bottles 250 mL. Non-injectable cap.
Description and technique
Technique
Melt the medium contained in the bottles in a water bath (100 °C), avoiding overheating, before pouring into Petri dishes when cooled to room temperature.
Once solidified on a flat surface, spread the plates by streaking methodology or by spiral method.
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Spread the plates by streaking methodology or by spiral method. Incubate the plates right side up aerobically at 25-30 °C for 48 h up to 5 days.
(Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications.)
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
Presentation
| Presentation | 10 prepared bottles, 250 mL |
|---|---|
| Filling | 200 ± 5 mL |
| Packaging | 1 box with 10 bottles 250 mL. Non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| Malt extract | 30.00 |
| Soy peptone | 3.00 |
| Chloramphenicol | 0.10 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Yellowish-brown |
|---|---|
| pH | 5.5 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Aerobiosis. Incubation at 25-30 °C, reading at 48-72 hours to 5 days.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 70%) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Inhibited |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M., L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- BALLOWS, HAUSLER, HERMAN, ISENBERG & SHADOMY (eds.) (1991) Manual of Clinical Microbiology. ASM. Washington.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed. APHA. Washington.
- FDA (Food and Drug Administration) (1978) Bacteriological Analytical Manual A.O.A.C. Washington.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- ISO 16000-17:2008 Indoor Air - Detection and enumeration of moulds - Culture Based method.
- RAPP, M. (1974) Indikator-Zusätze zur Keimdifferenzierung auf würze und Malzextrakt Agar. Milchwiss. 29:341-34.
- REIS, J. (1972) Ein selektives kulturmedium für der Nachweiss von Aspergillus flavus. Zbl. Bokt. Hyg. I. Abt. Orig. 220:564-566.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.