Ready-to-use media
Yeast Glucose Chloramphenicol Agar (YGC) ISO
Solid and selective medium for the isolation and enumeration of fungi in milk and dairy products according to ISO 7954 and FIL-IDF 94B standards.
- Cat. No.
- LC-4679
- Category
- Ready-to-use media
- Formats
- 2 formats
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- ISO 6611
This medium is recommended by the Federation International Laitière - International Dairy Federation (FIL-IDF) for the isolation and enumeration of fungi (moulds and yeast) in milk and dairy products. This medium has also been adopted by the DIN and ISO standards.
Key features
Applications
Selective enumeration and selective isolation of yeasts and molds.
Industry
Dairy products.
Standards
ISO 6611.
Description and technique
Description
This medium's selectivity is due to the bactericidal action of chloramphenicol which, due to its thermostability, may be sterilized with the medium in the autoclave. Also, due to the pH being neutral, the medium is able to be re-melted several times without affecting its stability, selectivity and efficacy. Re-melting and overheating may make the medium darker.
Technique
Melt the medium contained in the bottles in a water bath or in a microwave oven, avoiding overheating, before pouring into Petri dishes when cooled to room temperature.
Once solidified on a flat surface, spread the plates by streaking methodology or by spiral method.
Generally a stab inoculation method or pour plate method is used to inoculate the medium. Incubation is at 25 ± 1 °C for 5 days.
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
LC-4680, 250 mL bottles
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Once solidified on a flat surface, spread the plates by streaking method or by spiral method. This medium can be inoculated directly or after enrichment with broth.
Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc.
Place the plates upside down in the incubator, in aerobic conditions. Incubate the yeast and moulds for 48 hours-5 days at 25 ± 1 °C.
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-4679 | 10 prepared bottles, 125 mL | 100 ± 3 mL | 1 box with 10 bottles 125 mL. Non-injectable cap. |
| LC-4680 | 10 prepared bottles, 250 mL | 200 ± 5 mL | 1 box with 10 bottles 250 mL. Non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| Yeast extract | 5.0 |
| D(+)-Glucose | 20.0 |
| Chloramphenicol | 0.1 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 6.6 ± 0.2 at 25 °C |
Microbiological control
Spiral spreading: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 25 ± 1 °C, reading at 24-48-72 h to 5 days.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Inhibited |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
| Aspergillus nigerATCC 16404 | Good (≥ 50%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 50%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 50%) |
LC-4680, 250 mL bottles: microbiological control
Melting - pour plates - inoculation: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 25 ± 1 °C, reading at 24-48-72 h to 5 days.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 70%) |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| Bacillus cereusATCC 11778, WDCM 00001 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- DIN Standard 10186. Mikrobiologische Milch Untersuchung. Bestimmung der Anzahl von Hefen und Schimmelpilzen. Referenzverfahren.
- FIL-IDF 94B Standard (1991) Enumeration of yeast and moulds. Colony Count Technique at 25 °C.
- ISO 7954 Standard (1987) General guidance for enumeration of yeast and moulds - Colony count at 25 °C.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.