Ready-to-use media
Fecal Coliforms Agar (m-FC)
Selective and differential medium for coliform enumeration by the membrane filter technique.
- Cat. No.
- LC-4722
- Category
- Ready-to-use media
- Format
- 30 water plates, 55 mm
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 2 months
FC Agar and Broth are formulated according to Geldreich et al. to detect faecal coliforms in polluted water. The bile salts included in these media make them selective for enterobacteria, and also selective for coliforms due to the high incubation temperature: 44.5 ± 0.5 °C.
Key features
Applications
Selective enumeration of coliforms.
Industry
Water.
Presentation
30 prepared plates, 55 mm, for filtration purposes, with 9 ± 1 mL.
Packaging
1 box containing 5 plastic bags with 6 plates of 55 mm/bag.
Description and technique
Description
Freshly prepared medium has a red-garnet colour. Faecal coliform colonies are greenish-blue, and the medium also turns this colour. Other bacteria, when they grow, show red colonies, and then the medium turns red.
Technique
Essentially, the technique consists of filtering the test sample to be examined through a membrane filter of suitable porosity (0.22-0.45 µm), assisting the filtration by pressure or suction, so that the microorganisms are retained on the membrane. Remove the membrane carefully and aseptically and transfer it to the culture medium. Put the membrane over the agar, if using the solid medium, or over the impregnated pad if using the liquid version. Cover the Petri plates and incubate them at 36 ± 2 °C for 18-24 hours. After incubation, proceed with the counting of coliforms. Should total E. coli selectivity be desired, incubate at 44.5 ± 0.5 °C.
Presentation
| Presentation | 30 prepared plates, 55 mm, for filtration purposes |
|---|---|
| Filling | 9 ± 1 mL |
| Packaging | 1 box containing 5 plastic bags with 6 plates of 55 mm/bag. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptose | 10.0 |
| Yeast extract | 3.0 |
| Proteose peptone | 5.0 |
| Bile salts | 4.5 |
| Sodium chloride | 5.0 |
| Lactose | 12.5 |
| Aniline blue | 0.1 |
| Agar | 15.0 |
| Rosolic acid solution 1% | 10 mL |
Quality control
Physical/chemical control
| Color | Red / garnet |
|---|---|
| pH | 7.4 ± 0.2 at 25 °C |
Microbiological control
Membrane filtration / practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU for selectivity.
Aerobiosis. Incubation at 36 ± 2 °C, reading at 18-24 h.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Good (≥ 50%), blue colonies |
| Enterococcus faecalisATCC 29212, WDCM 00087 | Inhibited |
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 50%), blue colonies |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good - red colonies |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- GELDREICH, E.E., H.F. CLARK, C.B. HUFF and L.C. BEST (1965). Fecal-coliform-organism medium for the membrane filter technique. J. Am. Water Works Association (J.A.W.W.A.), 57:208-214.
- APHA-AWWA-WEF (1995) Standard Methods for the examination of water and wastewater. 19th ed. APHA. Washington.
- CLESCERI, L.S., A.E. GREENBERG and A.D. EATON (1998). Standard Methods for the examination of Water and Wastewater. 20th ed. APHA. Washington.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
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