Supplements and additives
Egg Yolk Emulsion 50%
Sterile egg yolk emulsion for microbiological media formulation.
- Cat. No.
- LC-5156
- Category
- Supplements and additives
- Format
- 500 mL
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 6 months
Egg emulsion for different culture media supplementation.
Key features
Applications
Detection of Bacillus cereus.
Industry
Food.
Presentation
1 prepared bottle, 1 L, with 500 ± 5 mL.
Packaging
1 box with 1 bottle 500 mL. Plastic screw inner cap.
Description and technique
Add aseptically to melted bottles of Bacillus cereus base medium (MYP Agar Base) cooled to 50 °C, before pouring into Petri dishes when cooled to room temperature.
Once solidified on a flat surface, spread the plates by streaking methodology or by spiral method. Incubate the plates right side up aerobically at 35-37 °C for 24-48 hours.
(Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc. This medium can be inoculated directly or after any enrichment broth.)
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Selective supplementation of the medium suppresses almost all the accompanying flora.
Each laboratory must evaluate the results according to their specifications.
Presumptive isolation of Bacillus sp. must be confirmed by further microbiological and biochemical tests.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
Presentation
| Presentation | 1 prepared bottle, 1 L |
|---|---|
| Filling | 500 ± 5 mL |
| Packaging | 1 box with 1 bottle 500 mL. Plastic screw inner cap. |
Composition
| Ingredient | g/L |
|---|---|
| Egg yolk | 500 mL |
| Sterile water | 500 mL |
| Sodium chloride | 4.25 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|
Microbiological control
Add 5 mL of product to 95 mL of Bacillus Cereus Agar Base.
Inoculate: practical range 100 ± 20 CFU, min. 50 CFU (productivity).
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 24-48 ± 2 h.
| Microorganism | Growth |
|---|---|
| Bacillus cereusATCC 11778, WDCM 00001 | Good |
Sterility control
Inoculate 10 mL of product in 100 mL THIO USP / TSB. Incubate and verify in TSA.
Incubation 7 days at 30-35 °C: NO GROWTH.
Bibliography
- SMITH, B.A. and BAIRD-PARKER, A.C. (1964) J. Appl. Bact. 27:28.
- VANDERZANT, C. and D.F. SPLITTSTOESSER (1992) Compendium of Methods for the Microbiological Examination of Foods. 3rd. Ed. APHA. Washington.
- CeNAN. (1982). Técnicas para el Análisis Microbiológico de Alimentos y Bebidas. Madrid.
- MOSSEL, D.A.A., KOOPMAN, M.J., JONGERIUS, E. (1967) Appl. Microbiol. 15, 650-653.
- NYGREN, B. (1962) Acta Path. Microbiol. Scand. 56, Suppl. 1-160.
- US-FDA Bacteriological Analytical Manual. BAM Media M51: Egg Yolk Emulsion, 50%.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.