Supplements and additives
Urea 40% Solution
Sterile additive for the urease test in microbiology.
- Cat. No.
- LC-5100
- Category
- Supplements and additives
- Format
- 100 mL flask
- Storage
- 8 to 14 °C
- Minimum guaranteed shelf life
- 12 months
- Standards
- ISO 19250ISO 6579
Aqueous urea solution 40%, sterilized by filtration and suitable to be used as an additive in culture media.
Key features
Applications
Differentiation of enterobacteria and confirmation of Salmonella.
Industry
Food, water.
Standards
ISO 19250, ISO 6579.
Presentation
1 prepared bottle, 125 mL, with 100 ± 3 mL.
Description and technique
It is supplied to be used with the dehydrated media Urea Agar according to Christensen and Urea Broth. It must be added to these media after the sterilization and with the media cooled to 50-55 °C.
Once it is added, do not reheat the media because urea is thermolabile and heating leads to its breakdown and ammonium liberation.
Presentation
| Presentation | 1 prepared bottle, 125 mL |
|---|---|
| Filling | 100 ± 3 mL |
| Packaging | 1 box with 1 bottle 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Urea | 400 |
| Distilled water | 1000 mL |
Quality control
Physical/chemical control
| Color | Colourless |
|---|
Microbiological control
Add the supplement into Urea Agar Base medium, for functionality testing.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 24-48 ± 2 h.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Urea negative |
| Proteus mirabilisATCC 43071 | Urea positive |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARK (1993) Handbook of Microbiological Media. CRC Press Inc. London.
- CHRISTENSEN W.B. (1946) Urea decomposition as means of differentiating Proteus and Paracolon cultures from each other and from Salmonella and Shigella types. J. Bact. 52:461.
- DIN Standard 10160. Untersuchung von Fleisch und Fleischerzeugnissen. Nachweis von Salmonellen. Referenzverfahren.
- DOWNES, F.P. & K. ITO (2001) Compendium of methods for the microbiological examination of foods. 4th ed. APHA. Washington DC. USA.
- EDWARDS & EWING (1962) Identification of Enterobacteriaceae. Burgess Pub. Co.
- FIL-IDF 93 Standard (2001) Milk and Milk products. Detection of Salmonella.
- ISO 6340 Standard (1995) Water Quality - Detection of Salmonella spp.
- ISO Standard 6579-1 (2017) Microbiology of food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.
- ISO 6785 Standard (2001) Milk and Milk products - Detection of Salmonella spp.
- ISO 21567 Standard (2004) Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Shigella spp.
- MARSHALL, R.T. (1992) Standard methods for the examination of dairy products. 16th ed. APHA. Washington DC. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
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