Dehydrated culture media
Eugon Agar
For the eugonic growth of most microorganisms.
- Cat. No.
- LC-1036
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Eugon Agar is a medium recommended for the growth of a wide variety of microorganisms. It yields a high level of growth of microorganisms (eugonic growth) even with bacteria which are more difficult to cultivate, such as Haemophilus, Neisseria, Pasteurella, Brucella, Lactobacillus, etc. It is very useful in medical bacteriology and food testing, such as cured meat, dairy products and other foods. Likewise, this medium is ideal for cultivating delicate pathogenic microorganisms and for obtaining high counts of bacterial cultures in the preparation of antigens and vaccines.
Key features
Applications
Enrichment (general use).
Industry
Food.
Preparation
45.4 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 118 °C for 15 minutes.
Principles and uses
L-Cystine and sodium sulfite are added to stimulate growth. Casein and soy peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. The addition of blood provides extra growth factors for fastidious microorganisms and the Polyenrichment Supplement (LC-6011) is a supplement specifically formulated for Neisseria, Francisella and Brucella.
The non-enriched medium is recommended for the rapid growth of lactobacilli linked to cured meat products, dairy products and other foods.
The addition of defibrinated blood, chocolated or not, permits the development of Histoplasma capsulatum and Nocardia. The medium also is used for the analysis of clinical materials such as blood and cerebrospinal or pleural fluids that generally contain pure cultures.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Clear amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 45.4 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 5.5 |
| Bacteriological agar | 15 |
| Casein peptone | 15 |
| L-Cystine | 0.7 |
| Sodium chloride | 4 |
| Sodium sulfite | 0.2 |
| Soy peptone | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 45.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 118 °C for 15 minutes. Cool to 45-50 °C and, if desired, aseptically add 5-10% sterile defibrinated sheep blood and two vials of Polyenrichment Supplement (LC-6011), previously reconstituted with 1 vial of Polyenrichment Restoring Solution (vial B). Homogenize gently and dispense into Petri dishes. Be careful to avoid bubble formation when adding the blood to the cooled medium and rotate the flask or bottle slowly to create a homogeneous solution.
Instructions for use
Inoculate sample and incubate at 35 ± 2 °C for 40-48 hours. Candida albicans and Aspergillus niger should be incubated at 30 ± 2 °C.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 40-48 h.
| Microorganism | Specification |
|---|---|
| Neisseria meningitidisATCC 13090 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Vera H.J. Bact. 54:14. 1947.
- Pelczar and Vera Milk Plant Monthly, 38-30. 1949.
- Frank J. Bact. 70:269, 1955.
- Ramos C., Mario "Manual of Milk and Lactides". Edition of Author, Berna 12:201. Mexico 6. D.F., 1976.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.