Dehydrated culture media
Trypticasein Glucose Extract Agar
For the plate count of bacteria in potable water, wastewater, air, milk and dairy products.
- Cat. No.
- LC-1041
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Trypticasein Glucose Extract Agar is used for the enumeration of bacteria from potable water, wastewater, air, milk and dairy products by the plate count method. This medium is recommended by APHA for the heterotrophic plate count procedure in testing bottled water.
Key features
Applications
Non-selective enumeration (general use).
Industry
Dairy products, water.
Preparation
24 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Casein peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. D-Glucose is a source of fermentable carbohydrate as the carbon and energy source. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Toasted |
| Color of the prepared medium | Clear amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 24 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Casein peptone | 5 |
| D-Glucose | 1 |
| Beef extract | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 24 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
Inoculate and incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Enterococcus faecalisATCC 11700 | Good growth | |
| Bacillus cereusATCC 11778 | Good growth | |
| Salmonella typhimuriumATCC 14028 | Good growth | |
| Escherichia coliATCC 25922 | Good growth | |
| Staphylococcus aureusATCC 25923 | Good growth | |
| Pseudomonas aeruginosaATCC 27853 | Good growth | Pigment production |
Bibliography
- Standard Methods for the Examination of Water and Wastewater. 11th Edition APHA Inc. New York, 1960.
- Standard Methods for the examination of dairy products, 16th ed. American Public Health Association; Washington D.C. Marshall, R.T. (1993).
- Standard Methods for the examination of water and wastewater 18th ed. American Public Health Association, Washington D.C. 1992.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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