Ready-to-use media
Luria Agar (Miller’s LB Agar) with Ampicillin (100µg/ml)
Solid selective medium, recommended for molecular genetic studies with resistant Escherichia coli.
- Cat. No.
- LC-997
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
To a classic Miller’s medium, an antibiotic (ampicillin) has been added for molecular genetic studies in resistant E. coli.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
Technique
Dilute and prepare samples and volumes as necessary according to specific protocols, established regulations, official directives and/or expected results. Each technician must evaluate the results according to the specifications established in his laboratory.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
| Ampicillin | 0.100 |
| Agar | 15.000 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Spiral spreading: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 35 ± 2 °C. Reading at 24 hours.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Inhibited |
| Escherichia coli DH5 alpha + pUC19 | Good (≥ 50%) |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
Sterility control
Incubation 48 h at 30-35 °C and 48 h at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- AUSUBEL, F.M., R. BRENT, R.E. KINGSTON, D.D. MOORE, J.G. SEIDMAN, J.A. SMITH & K. STRUHL (1994) Current protocols in molecular biology. Greene Pub. Assoc. Inc. Brooklyn. NY.
- GHERNA, R., P. PIENTA & R. COTE (Eds.) 1992. ATCC Catalogue of Bacteria and Bacteriophages. Media #1065, #1226, #1226, #1235, #1236, #1315, #1364. American Type Culture Collection. Rockville. MD. USA.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- LURIA, S.E. & J.W. BURROUS (1955) Hybridization between Escherichia coli and Shigella. J. Bacteriol. 74:461-476.
- LENNOX, E.S. (1955) Transduction of linked genetic character of the host bacteriophage P1. Virology 1:190-206.
- SAMBROOK, J., E.F. FITSCH & T. MANIATIS (1989) Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory. Cold Spring Harbor. NY.
- MILLER, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
- MILLER, J.H. (1992) A short course in bacterial genetics: A laboratory manual and handbook for Escherichia coli and related bacteria, p. 194-195. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
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