Ready-to-use media
M-Green Yeast and Mould Agar
Solid selective culture medium for enumeration of fungi according to ISO Standard 10718:2002. Specially for brewery industries.
- Cat. No.
- LC-988
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
This classical formulation, used by the food industry for the detection and enumeration of yeasts and moulds by the membrane filter method, was adopted by ISO for application in cork stoppers for alcoholic or non-alcoholic beverages in the 10718:2002 Standard. The composition of the culture broth includes bromocresol green indicator, which facilitates the visualization and counting of fungal colonies. The fungal colonies are green due to the diffusion of the dye into the colonies (alkaline reaction). The end products of the microbial growth diffuse into the medium, reducing the pH and turning the indicator to yellow (acid reaction). Bacterial growth is inhibited by the acid pH.
Key features
Applications
Detection of yeasts and molds.
Industry
Alcoholic beverages.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
Technique
Roll the membrane filter used to filter the test sample onto the surface of the medium, avoiding the formation of air bubbles. Incubate the plates at 30 ± 2 °C for 3 days. Observe and count the colonies on each plate at least every 24 hours.
After incubation, colonies appearing on the filter surface can be counted. Mould colonies generally appear green and filamentous, whereas yeast colonies are green and opaque.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Dextrose | 50.000 |
| Peptone | 10.000 |
| Yeast extract | 9.000 |
| Magnesium sulfate | 2.100 |
| Potassium phosphate | 2.000 |
| Diastase | 0.050 |
| Thiamine | 0.050 |
| Bromocresol green | 0.026 |
| Agar agar | 15.000 |
Quality control
Physical/chemical control
| Color | Green |
|---|---|
| pH | 4.6 ± 0.2 at 25 °C |
Microbiological control
Spiral spreading / MF methods: practical range 100 ± 20 CFU, min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 22.5 ± 2.5 °C. Reading at 24-72 h for bacteria and 3-5 days for yeasts and moulds.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good |
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited - poor |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press. London.
- ISO Standard 10718:2002 Cork stoppers. Enumeration of colony-forming units of yeasts, moulds and bacteria capable of growth in an alcoholic medium.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
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