Ready-to-use media
M-YSG Broth
Liquid medium used for the detection and enrichment of Alicyclobacillus in fruit juices and other acidic foods, according to IFU Standard Method No. 12.
- Cat. No.
- LC-4104
- Category
- Ready-to-use media
- Format
- 50 tubes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 3 months
- Standards
- IFU Method No. 12
Alicyclobacillus has emerged as a food spoiling microorganism of major significance in the fruit juice industry (Baumgart & Menje, 2000). Spoilage is generally manifested as the formation of off-flavours and odours from compounds such as guaiacol and the halogenated phenols. The economic impact of spoilage can be very high; to date, no human risk has been associated with the consumption of such juices and other food products containing Alicyclobacillus bacteria.
Key features
Applications
Growth of Alicyclobacillus.
Industry
Juices.
Standards
IFU Method No. 12.
Presentation
50 tubes, 3 mL (capacity), with 2 ± 0.1 mL.
Description and technique
Description
An acidic environment is required to grow alicyclobacilli, and YSG medium supports the growth of all currently known species of Alicyclobacillus (A. acidocaldarius, A. acidoterrestris, A. cycloheptanicus and A. hesperidum). The medium complies with the Standard IFU Method for the detection of taint producers in fruit juices.
The low pH value of the medium, in combination with the high incubation temperature, inhibits the growth of contaminating microbiota. K Agar when incubated at 45 °C predominantly supports the growth of A. acidoterrestris and limited growth of other species of the same genus. Therefore, K Agar is used to detect predominantly A. acidoterrestris strains.
Technique
If the membrane filtration method is used, impregnate a sterile pad with the medium, place the membrane filter on the pad and incubate. Plates must be protected against dehydration.
According to the internal regulations or methodologies of each laboratory, temperature and incubation time can vary.
Note: The material and quality of the membrane filter significantly affect the recovery of microorganisms.
Presentation
| Presentation | 50 tubes, 3 mL (capacity) |
|---|---|
| Filling | 2 ± 0.1 mL |
| Packaging | 1 box with 50 tubes manufactured from non-toxic polypropylene. Suitable for cultivation and transport of biological material. |
Composition
| Ingredient | g/L |
|---|---|
| Yeast extract | 2.0 |
| Dextrose | 1.0 |
| Soluble starch | 2.0 |
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 3.7 ± 0.2 at 25 °C |
Microbiological control
Membrane filtration / practical range 100 ± 20 CFU, min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 50-55 °C for up to 3-5 days.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Inhibited |
| Alicyclobacillus acidoterrestrisATCC 49025 | Good |
| Bacillus cereusATCC 11778, WDCM 00001 | Inhibited |
| Alicyclobacillus acidocaldarius NCIMB 11725 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- BAUMGART, J. (2003) Media for detection and enumeration of Alicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius in foods. In Handbook of Culture Media for Food Microbiology. J.E.L. Corry et al. (Eds.) Elsevier Sci B.V. Amsterdam.
- BAUMGART, J. & S. MENJE (2000) The impact of Alicyclobacillus acidoterrestris on the quality of juices and soft drinks. Fruit Processing 7:251-254.
- CERNY, G., W. HENNLICH & K. PORALLA (1984) Fruchtsaftverderb durch Bazillen: Isolierung und Charakterisierung des Verderberregers. Z. Lebens. Unter Forsch. 179:224-227.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- IFU Standard (2004) Method No. 12 on the detection of taint producing Alicyclobacillus in fruit juices. Rev. March 2007.
- WITTHUHN, R.C., W. DUVENAGE & P.A. GOUWS (2007) Evaluation of different growth media for the recovery of the species of Alicyclobacillus. Letters Appl. Microbiol. 45:224-229.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.