Ready-to-use media
Plate Count Agar (PCA) ISO
Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO 4833, 8552 & 17410 Standards and IFU No. 6.
- Cat. No.
- LC-4105
- Category
- Ready-to-use media
- Format
- 20 tubes
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- ISO 11133ISO 4833
The Plate Count Agar formulation is according to that of Buchbinder et al. as recommended in their study of media for the plate count of microorganisms.
Key features
Applications
Non-selective enumeration of aerobes.
Industry
Dairy products, food.
Standards
ISO 11133, ISO 4833.
Presentation
20 tubes, 17 x 145 mm, with 20 ± 0.3 mL.
Description and technique
Description
The original formulation of the standardized agar for dairy microbiology has been modified in order to avoid the addition of milk. This new composition allows the growth of most microorganisms without any further additions.
This medium's formulation is equivalent to that described by the "Standard Methods for the Examination of Dairy products", the USP's "Tryptone Glucose Yeast Agar", the "Deutsche Landwirtschaft" and to the APHA, ISO and AOAC's Plate Count Agar. This is the medium of choice for the plate count of any type of sample.
Technique
To use, the contents of the tube should be poured into plates. The melting of the culture medium should be carried out according to the manufacturer's instructions, either in a water bath (100 °C) or microwave oven.
The incubation time and temperature depend on the type of microorganism under study. For a general aerobic count, incubate for 3 days at 30 °C, taking readings after 24, 48 and 72 hours.
After incubation, enumerate all the colonies that have appeared onto the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Presentation
| Presentation | 20 tubes, 17 x 145 mm |
|---|---|
| Filling | 20 ± 0.3 mL |
| Packaging | 17 x 145 mm glass tubes, ink labelled, metal non-injectable cap. 20 tubes per box. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone from casein | 5.0 |
| Yeast extract | 2.5 |
| D(+)-Glucose | 1.0 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 30 ± 1 °C, reading at 24-48-72 h.
| Microorganism | Growth |
|---|---|
| Bacillus subtilisATCC 6633, WDCM 00003 | Good (≥ 70%) |
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 70%) |
| Stph. aureusATCC 25923, WDCM 00034 | Good (≥ 70%) |
| Listeria monocytogenesATCC 35152 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- BUCHBINDER, L., Y. BARIS & L. GOLDSTEIN (1953) Further studies on new milk-free media for the standard plate count of dairy products. Am. J. Public Health 43:869-872.
- CLESCERI, L.S., A.E. GREENBERG and A.D. EATON (1998) Standard Methods for the Examination of Water and Wastewater. 20th ed., APHA, AWWA, WPCF. Washington.
- DIN 10192 (1971) Prüfungsbestimmungen für Milch und Milcherzeugnisse. Deutsche Landwirtschaft, Fachbereit und Ernahrung.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed., APHA, Washington.
- FIL/IDF Standards 3 (1958), 100, 101 (1981), 109 (1982) and 132 (2004).
- HORWITZ, W. (2000) Official Methods of Analysis. AOAC International. Gaithersburg.
- IFU Method No. 6 (1996) Mesophilic, thermoduric and thermophilic bacteria: Spores Count. D-1 Mesophilic Aerobic Sporeforming bacteria: Spores count.
- ISO 4833 (2003) Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of microorganisms. Colony count technique at 30 °C.
- ISO 8552 (2004) Milk - Estimation of psychrotrophic microorganisms. Colony count technique at 21 °C (Rapid method).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 17410 (2001) Horizontal method for the enumeration of psychrotrophic microorganisms.
- MARSHALL, R.T. (1992) Standard Methods for the Examination of Dairy Products. 16th ed. APHA. Washington.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos, S.A. Madrid.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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