Ready-to-use media
Malt Extract Broth Acidified (pH 4,3)
Liquid medium for the cultivation of yeasts and moulds by membrane filtration (MF) methods.
- Cat. No.
- LC-4103
- Category
- Ready-to-use media
- Format
- 50 tubes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 3 months
This formulation of the classic Malt Extract Broth is according to Reiss's modification, where the pH has been lowered to obtain greater selectivity of the medium. The pH should not be lowered below 3.5, since these conditions make growth difficult and delay it a lot. The culture medium should not overheat.
Key features
Applications
Growth of yeasts and molds.
Industry
Water.
Presentation
50 tubes, 3 mL (capacity), with 2 ± 0.1 mL.
Packaging
1 box with 50 tubes manufactured from non-toxic polypropylene. Suitable for cultivation and transport of biological material.
Description and technique
Technique
If the membrane filtration method is used, impregnate a sterile pad with the medium, place the membrane filter on the pad and incubate. Plates must be protected against dehydration.
According to the internal regulations or methodologies of each laboratory, temperature and incubation time can vary.
Note: The material and quality of the membrane filter significantly affect the recovery of microorganisms.
Presentation
| Presentation | 50 tubes, 3 mL (capacity) |
|---|---|
| Filling | 2 ± 0.1 mL |
| Packaging | 1 box with 50 tubes manufactured from non-toxic polypropylene. Suitable for cultivation and transport of biological material. |
Composition
| Ingredient | g/L |
|---|---|
| Malt extract | 17.0 |
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 4.2 ± 0.2 at 25 °C |
Microbiological control
Membrane filtration / practical range 100 ± 20 CFU, min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 20-25 °C. Reading at 2-3 days up to 5 days.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Saccharomyces cerevisiaeATCC 9763 | Good |
| Acetobacter acetiATCC 15973 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press. Boca Raton. Fla. USA.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- RAPP, M. (1974) Indikator-Zusätze zur Keimdifferenzierung auf Würze und Malzextrakt Agar. Milchwissenschaft 29:341-344.
- REIS, J. (1972) Ein selektives kulturmedium für der Nachweiss von Aspergillus flavus. Zbl. Bakt. Hyg. I. Abt. Orig. 220:564-566.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.