SCDLP 80 Broth ISO

The composition of this medium corresponds to the classical TSB (Trypticase Soy Broth) formulation with the addition of lecithin and polysorbate 80. The relatively high casein peptone content provides optimal conditions for the spore germination and regeneration of even damaged microorganisms. Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and, with lecithin, ethanol. Casein peptone and soy peptone provide the nitrogen source, vitamins and growth factors, sodium chloride maintains the osmotic balance and dipotassium hydrogen phosphate acts as a buffer.

Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.

Preparation

Suspend 38.0 grams of the medium in a liter of distilled water. Dissolve by heating with frequent agitation. Boil for 1 minute. Distribute in the appropriate containers and sterilize at 121 °C for 15 minutes.

Instructions for use

1. Add 1 mL or 1 g of the sample to 9 mL of the enrichment broth.
2. Incubate the prepared suspension at 32.5 ± 2.5 °C for at least 20 hours.
3. Spread an aliquot of the incubated enrichment broth on a non-selective agar plate.
4. Incubate the plate at a temperature of 32.5 ± 2.5 °C for 48-72 hours.
5. Carry out the specific procedures for the identification of P. aeruginosa, E. coli, S. aureus and C. albicans.

• ISO 18415:2017 Cosmetics - Microbiology - Detection of specified and non-specified microorganisms.