Dehydrated culture media
Liver Veal Agar
For the cultivation of anaerobic organisms.
- Cat. No.
- LC-2208
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 10 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- BAM
Liver Veal Agar is recommended by the FDA Bacteriological Analytical Manual and the Compendium of Methods for the Microbiological Examination of Foods. It is designed to support the growth of various microorganisms, in particular those relevant to food safety assessments. The composition of this medium is tailored to provide essential nutrients, which makes it an effective choice for microbiological analysis.
Key features
Applications
Growth of anaerobes and Clostridium.
Industry
Food.
Standards
BAM.
Preparation
97 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The extracts, peptones, gelatine and casein provide a substantial amount of nitrogen and amino acids, which are essential for microbial growth. Conversely, dextrose serves as the primary carbon source, promoting metabolic activity. Sodium chloride maintains osmotic balance, ensuring optimal growth conditions.
Soluble starch is an essential component in microbiological analysis, as it supports the growth of anaerobic bacteria, a vital element in comprehensive food safety testing. Bacteriological agar also serves as a solidifying agent, ensuring a reliable and stable environment for microbial colonies.
Technical specifications
| Solubility | With residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Light yellow-amber |
| Color of the prepared medium | Brown opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 97 g |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 13 |
| Casein peptone | 4.6 |
| D(+) Glucose | 5 |
| Gelatin peptone | 20 |
| Meat peptone | 14 |
| Sodium chloride | 5 |
| Starch | 10 |
| Tryptone | 8 |
| Yeast extract | 7 |
| Sodium nitrate | 2 |
| Liver extract | 5.4 |
| Meat extract | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 97 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
If desired, add Egg Yolk Emulsion (LC-5156) for the cultivation of anaerobic organisms.
Instructions for use
- Inoculate and incubate at 35-37 °C for 18-48 hours under appropriate atmospheric conditions.
Quality control
Microbiological test
Incubation conditions: 37 °C, anaerobic conditions / 18-48 h.
| Microorganism | Specification |
|---|---|
| Clostridium perfringensATCC 12916 | Good growth |
| Clostridium perfringensATCC 13124 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
Bibliography
- U.S. Food and Drug Administration. 2001. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md.
- Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C
What you receive
The product
Shipped under controlled temperature when the product requires it.
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