Dehydrated culture media
Trypticasein Soy Broth (TSB) With Tween 80
For the determination of sanitization efficiency.
- Cat. No.
- LC-2157
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Trypticasein Soy Broth (TSB) with Tween 80 is used for determining the efficiency of disinfection of containers, surfaces, water, cosmetics, etc. and other products of sanitary importance. It can also be utilized for the detection and enumeration of microorganisms from water-insoluble products and fatty products containing preservatives or antimicrobials.
Key features
Applications
Detection (general use).
Preparation
35.7 g in one liter of distilled water; heat slightly until complete dissolution, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Containing two peptones as rich nitrogen sources, obtained by the enzymatic hydrolysis of casein and soy proteins, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. Besides nitrogen, peptones also provide vitamins, minerals and amino acids essential for microbial growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Tween 80 and soya lecithin neutralize quaternary ammonium compounds and parahydroxybenzoates.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Light beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 35.7 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 2.5 |
| Dipotassium phosphate | 2.5 |
| Pancreatic digest of casein | 17 |
| Papainic digest of soy bean | 3 |
| Sodium chloride | 5 |
| Tween 80 | 5 |
| Soy lecithin | 0.7 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 35.7 grams of the medium in one liter of distilled water. Mix well. Heat slightly until complete dissolution of the medium is achieved. Dispense in the appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Samples should be collected from test areas before and after the treatment with disinfectant in order to evaluate cleaning procedures in environmental sanitation.
- Inoculate the sample and incubate at a temperature of 30-35 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 30-35 °C / 18-24 h.
Inoculation conditions: 50-100 CFU.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Pseudomonas aeruginosaATCC 27853 | Good growth |
| Staphylococcus aureusATCC 6538 | Good growth |
| Escherichia coliATCC 8739 | Good growth |
| Pseudomonas aeruginosaATCC 9027 | Good growth |
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Food, 4th ed. ASM. Washington. DC.
- Murray PR, Baron, Pfaller, and Yolken (Eds.), 2003, In Manual of Clinical Microbiology, 8th ed., ASM, Washington, D.C
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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