Supplements and additives
Selective Supplement MUP
For the detection of viable bifidobacteria.
- Cat. No.
- LC-6074
- Category
- Supplements and additives
- Format
- 10 vials, each for 500 mL of medium
- Storage
- 2 to 8 °C
- Minimum guaranteed shelf life
- 15 months
- Minimum order
- 20 packs
- Standards
- ISO 29981
TOS Propionate Agar is a medium according to ISO 29981 for the direct detection of viable bifidobacteria. The method is applicable to milk products such as fermented and non-fermented milks, milk powders, infant formulae and starter cultures, where these microorganisms are present and viable, and in combination with other lactic acid bacteria.
Key features
Applications
Detection of Bifidobacterium.
Industry
Dairy products.
Standards
ISO 29981.
Preparation
Reconstitute each vial with 5 mL of warm sterile distilled water; add 2 vials to 990 mL of TOS Propionate Agar Base.
Principles and uses
Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Magnesium sulfate allows the detection of small numbers or injured bifidobacteria. Ammonium sulfate acts as a nitrogen source during growth. Potassium phosphates act as a buffer system. L-cysteine hydrochloride is the reducing agent. Transgalacto-oligosaccharide (TOS) enhances the growth of bifidobacteria used in dairy products, as it is a specific growth factor for all bifidobacteria, whereas other lactic acid bacteria cannot utilize this saccharide. Sodium propionate inhibits the accompanying flora. The antibiotic mupirocin lithium salt (MUP) inhibits the growth of most lactic acid bacteria commonly used in fermented and non-fermented dairy products.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Lyophilized tablet |
| Color of the dehydrated medium | N/A |
| Color of the prepared medium | Slightly white |
| Final pH (25 °C) | N/A |
| Storage | 2 to 8 °C |
Formula
| Ingredient | mg/vial |
|---|---|
| Lithium-mupirocin (MUP) | 25 |
Preparation and use
Preparation
Aseptically reconstitute each vial with 5 mL of warm sterile distilled water. Mix gently until complete dissolution and aseptically add 2 vials to 990 mL of TOS Propionate Agar Base (LC-2011). Mix well and distribute into sterile containers.
Instructions for use
For the enumeration of presumptive bifidobacteria according to ISO 29981:
- Prepare the initial sample according to the raw material.
- Perform the decimal dilutions.
- Inoculate 1 mL of each dilution on empty Petri dishes. Pour 12-15 mL of the prepared propionate TOS medium. Mix the content without incorporating air.
- When the medium solidifies, incubate the plates in an anaerobic incubator at 37 °C for 72 ± 3 h.
- Counting and confirmation of colonies.
Quality control
Microbiological test
Incubation conditions: 37 °C / 72 ± 3 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Bifidobacterium breveATCC 15700 | Good growth | White colonies |
| Streptococcus salivarius subsp. thermophilusATCC 19258 | Total inhibition | |
| Lactobacillus caseiATCC 393 | Total inhibition |
Bibliography
- ISO 29981/IDF 220. Milk products - Enumeration of presumptive bifidobacteria - Colony count technique at 37 °C (2010).
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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