Supplements and additives
Vancomycin Supplement
Sterile selective supplement used for isolation and enrichment of Cronobacter sakazakii.
- Cat. No.
- LC-6072
- Category
- Supplements and additives
- Format
- 10 vials, each for 10 mL of medium
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- ISO 22964
Selective supplement added to the medium CSB, in order to inhibit most Gram-positive flora present in the sample.
Key features
Applications
Selective enrichment of Cronobacter.
Industry
Food.
Standards
ISO 22964.
Presentation
10 freeze-dried vials with 3 ± 0.1 g.
Description and technique
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Reconstitute the vial with a sterile diluent in aseptic conditions and add it to 500 mL of medium base cooled to room temperature.
Do not overheat once supplemented.
Pour the complete medium into suitable containers.
Inoculate and incubate according to standards and specifications.
Presumptive isolation of the required microorganism must be confirmed by further tests.
Presentation
| Presentation | 10 freeze-dried vials |
|---|---|
| Filling | 3 ± 0.1 g |
| Packaging | 22 ± 0.25 x 55 ± 0.5 mm glass vials, tag labelled, white plastic cap - 10 vials per box. |
Composition
| Ingredient | g/vial |
|---|---|
| Vancomycin HCl | 0.005 |
Reconstitute the original freeze-dried vial by adding 6 mL of sterile distilled water.
Note: each vial is sufficient to supplement 500 mL of medium base (CSB).
Quality control
Physical/chemical control
| Color | White-grey |
|---|
Microbiological control
Reconstitute 1 vial as indicated in Composition; shake and dissolve completely.
Add 1 vial to 500 mL of medium base. DO NOT HEAT once supplemented.
Aerobiosis. Incubation at 41.5 ± 1 °C, reading at 24 ± 3 h.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Inhibition (partial to complete) |
| Cr. sakazakiiATCC 29544, WDCM 00214 + ATCC 25923, WDCM 00034 | Good, yellow medium |
| Cr. muytjensiiATCC 51329, WDCM 00213 + ATCC 25923, WDCM 00034 | Good, yellow medium |
Sterility control
Study 5 vials: reconstitute and dissolve each one in 100 mL of TSA + neutralizers, then pour into 90 mm plates.
Incubation 24 hours at 30-35 °C and 72 hours at 20-25 °C: NO GROWTH.
Incubation 7 days at 32.5 ± 2 °C and 7 days at 22.5 ± 2 °C: NO GROWTH.
Bibliography
- ISO 22964 (2017) Microbiology of the food chain - Horizontal method for the detection of Cronobacter spp.
- IVERSEN C., A. LEHNER, N. MULLANE, J. MARUGG, S. FANNING, R. STEPHAN, and H. JOOSTEN (2007) The identification of Cronobacter spp. (Enterobacter sakazakii). J. Clin. Microbiol. 45: 3814-3816.
- IVERSEN, C., P. DRUGGAN, S. SCHUMACHER, A. LEHNER, C. FEER, K. GSCHWEND, H. JOOSTEN and R. STEPHAN (2008) Development of a Novel Screening Method for the Isolation of "Cronobacter" spp. (Enterobacter sakazakii). Appl. Environm. Microbiol. 74: 2550-2553.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
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