PCR accessories
Uracil-DNA Glycosylase (UDG)
Recombinant enzyme that prevents carry-over contamination in dUTP-based PCR
- Cat. No.
- M3096
- Category
- PCR accessories
- Formats
- 2 formats
- Manufacturer
Recombinant uracil-DNA glycosylase from E. coli (26 kDa) that removes uracil from single- and double-stranded DNA. When every PCR in the lab uses dUTP in place of dTTP, a short UDG treatment of each new reaction destroys contaminating amplicons from earlier runs before amplification starts. The enzyme is inactivated at 95 °C and does not act on oligonucleotides of 6 bases or fewer.
Key features
Carry-over protection
1 unit per reaction for 10 minutes at 15-25 °C before PCR degrades uracil-containing amplicons.
Simple inactivation
A 10-minute incubation at 95 °C inactivates the enzyme.
Primers stay intact
Uracil is not removed from oligomers of 6 bases or fewer.
Reusable products
dU-containing products work as hybridisation targets and sequencing templates and can be cloned into UNG-deficient hosts.
Applications
- Prevention of carry-over contamination in PCR
- Decontamination of reactions set up with dUTP
- Removal of uracil from single- and double-stranded DNA
Technical details
| Concentration | 10 U/µL |
|---|---|
| Source | E. coli, recombinant |
| Unit definition | 1 U releases 60 pmol of uracil per minute from uracil-containing double-stranded DNA |
| Storage buffer | 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 0.1 mM EDTA, 50% glycerol |
| Dosage | 1 U per reaction, 10 minutes at 15-25 °C before PCR |
| Requirements | dUTP (200-600 µM) in place of dTTP; at 500-600 µM dUTP raise MgCl2 by 2.5 mM |
| Inactivation | 95 °C for 10 minutes |
| Storage | -20 °C; PCR products at +2/+8 °C for a few hours or at -20 °C long term |
| Shipping | On wet ice |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.