qPCR kits for food and environment
ViableCell Reagent for qPCR
Reagent for viability PCR.
- Cat. No.
- LC-6502
- Format
- 2 x 1 mL tubes
- Storage
- 2 to 8 °C
- Minimum guaranteed shelf life
- 4 months
Reagent intended to neutralize DNA from dead cells and/or free DNA that could lead to false positives in PCR-based detection techniques. This reagent cannot cross intact cell membranes associated with cell viability.
Key features
Applications
Detection and selective enumeration (general use).
Industry
Food.
Presentation
2 microtubes.
Description and technique
Description
The results obtained from PCR in samples treated with the reagent are indicators of the presence of viable cells.
Technique
Follow the product instructions. Each laboratory must evaluate the results according to specifications. Proceed according to directives and regulations.
This reagent is shipped at room temperature; the shipment at room temperature does not affect the performance of the kit.
Once received, store the product at 2-8 °C; under these conditions and with proper handling, the kit performance is guaranteed at least until the expiration date.
Presentation
| Presentation | 2 tubes (microtubes) |
|---|---|
| Packaging | 2 microtubes per box |
Composition
| Synonym | 3-Amino-8-azido-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium dichloride |
|---|---|
| Formula | C27H32N6Cl2 |
| Molecular weight | 511.49 |
| CAS No. | 91416-20-5 |
Quality control
Control PCR
vPCR: Salmonella spp. (> 1·10⁶) with and without heat treatment (inactivated at 85 °C, 30 min).
Total qPCR cycles: 45.
| Test | Specification |
|---|---|
| Purity | > 94% |
| Abs 480 nm (100-140 µM) | 0.39-0.53 |
| vPCR functionality test | delta Ct (reagent-dead, live) > 12 Ct |
Sterility control
Incubation 48 h at 30-35 °C and 48 h at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in the same conditions.
Bibliography
- Fittipaldi M, Nocker A, Codony F. Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification. J Microbiol Methods. 2012 Nov;91(2):276-89. doi: 10.1016/j.mimet.2012.08.007. Epub 2012 Aug 23. PMID: 22940102.
- Codony F, Dinh-Thanh M, Agustí G. Key Factors for Removing Bias in Viability PCR-Based Methods: A Review. Curr Microbiol. 2020 Apr;77(4):682-687. doi: 10.1007/s00284-019-01829-y. Epub 2019 Dec 6. PMID: 31811375.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.