Nucleases and proteases
DNase I (EC 3.1.21.1), bovine pancreas
Lyophilised endonuclease from bovine pancreas, ≥3,000 U/mg (Kunitz)
- Cat. No.
- M3028
- Category
- Nucleases and proteases
- Formats
- 2 formats
- Manufacturer
DNase I from bovine pancreas, chromatographically purified and supplied as a salt-free lyophilised powder with a minimum activity of 3,000 Kunitz units per mg. It cleaves single- and double-stranded DNA and chromatin into oligo- and mononucleotides with 5'-phosphate and 3'-OH ends. It is not guaranteed RNase-free: before use in RNA preparation it must undergo an RNase inactivation treatment.
Key features
High specific activity
At least 3,000 U/mg (Kunitz), for efficient digestion with small amounts of enzyme.
Tunable cleavage
Mg2+ gives single-strand nicks, Mn2+ gives double-strand breaks; maximum activation with Mg2+ plus Ca2+.
Lyophilised format
Salt-free powder, soluble at 5 mg/mL in 150 mM NaCl, for custom stock solutions.
Versatile use
From tissue dissociation to nick translation, footprinting and chromatin studies.
Applications
- Preventing cell clumping during tissue dissociation
- Removing contaminating DNA from RNA preparations before RT-PCR, after RNase inactivation
- DNA removal during single-cell isolation
- Random nicking for nick translation
- Footprinting assays
- Chromatin studies
- DNA digestion
Technical details
| Source | Bovine pancreas |
|---|---|
| Form | Salt-free lyophilised powder, chromatographically purified |
| Activity | ≥3,000 U/mg (Kunitz) |
| Molecular weight | About 31 kDa |
| Optimal conditions | pH 7.8; activated by Mg2+ and Ca2+; 5 mM Ca2+ protects against proteolysis |
| Inhibitors | EDTA, EGTA, DTT, SDS, beta-mercaptoethanol, salt above 100 mM |
| Inactivation | Heat (99 °C for 10 minutes), also combined with EDTA at about a 1:1 molar ratio to divalent cations |
| RNase | Not guaranteed RNase-free: pretreatment required for RNA work |
| CAS | 9003-98-9 |
| Storage | -20 °C; stock solutions (at least 1 mg/mL) stable for over a year at -20 °C |
| Shipping | On wet ice |
| Quality control | Activity ≥3,000 U/mg; RNase test with tritium-labelled RNA (maximum 3% release) |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.