Ready-to-use media
Phosphate Buffered Saline (PBS)
Buffered diluent for multiple uses in microbiology.
- Cat. No.
- LC-4015
- Category
- Ready-to-use media
- Formats
- 2 formats
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 6 months
Buffered diluent used in routine processes of preparation of dilution banks for several microbiological tests.
Key features
Applications
Diluent (general use).
Industry
General cultivation.
Description and technique
Inoculate according to final purpose, samples and validated methods.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-4015 | 20 tubes, 16 x 113 mm | 9 ± 0.1 mL | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metallic cap. |
| LC-4668 | 10 prepared bottles, 125 mL | 100 ± 3 mL | 1 box with 10 bottles 125 mL. Non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| Potassium dihydrogen phosphate | 0.20 |
| Disodium hydrogen phosphate | 1.15 |
| Sodium chloride | 8.00 |
| Potassium chloride | 0.20 |
Quality control
Physical/chemical control
| Color | Colourless |
|---|---|
| pH | 7.2 ± 0.1 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Subculture onto appropriate culture media after holding at 20-25 °C for 45 min to 1 h.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good. Recovery ± 30% T0 (original enumeration) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good. Recovery ± 30% T0 (original enumeration) |
LC-4668, bottles: microbiological control
Prepare tubes / inoculate 10³-10⁴ (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Aerobiosis. Incubation at 35 ± 2 °C. Reading at 48 h.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good. Recovery ± 30% T0 (original enumeration) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good. Recovery ± 30% T0 (original enumeration) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1997) Handbook of microbiological media. CRC Press. Boca Raton. Fla. USA.
- Dulbecco and Vogt (1954) J. Exp. Med. 99. 167-182.
- Paul J. (1965) ‘Cell and Tissue Culture’ 3rd ed, Livingstone Ltd., London.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.