Ready-to-use media
Thyoglicollate Fluid Medium EP/USP/ISO
Fluid medium used for sterility testing according to the Eur. Pharm., USP, FDA, and for the cultivation of microaerophilic and anaerobic organisms.
- Cat. No.
- LC-4004
- Category
- Ready-to-use media
- Format
- 20 tubes
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- European PharmacopoeiaISO 7937USP
Thioglycolate Fluid Medium is a standard medium formulated and recommended by the European Pharmacopoeia, USP, APHA and FDA. The reducing agents thioglycolate and L-cystine ensure anaerobiosis which is adequate even for fastidious anaerobes.
Key features
Applications
Confirmation of Clostridium and enrichment (general use).
Industry
Final product quality control, food, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, ISO 7937, USP.
Presentation
20 tubes, 16 x 113 mm, with 9 ± 0.1 mL.
Description and technique
Description
The -SH groups of these substances also inactivate arsenic, mercury and other heavy metal compounds. Thioglycolate media are thus suitable for the examination of materials which contain heavy metals or heavy metal preservatives.
In the present formulation a special agar with a high viscosity but a very low turbidity is used. A very slow cooling is recommended to prevent stratification. The higher viscosity of the fluid thioglycolate medium prevents rapid uptake of oxygen. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes colour to pink.
Technique
For the inoculation of tubes, follow the standard laboratory method or the applicable norms (stab inoculation, loop inoculation, dilution banks, etc.).
The methodology is according to the standard methods described in the Pharmacopoeia.
Inoculate the culture medium with the sample material taking care that the sample reaches the bottom of the tube.
Incubate for at least 14 days at the optimal temperature. Anaerobes grow in the lower part of the culture medium container.
Precautions and limitations of the procedure:
- Store the prepared medium away from light at room temperature.
- If more than 30% of the medium is pink prior to use, reheat once at 100 °C to drive off absorbed oxygen.
- Do not reheat the medium more than once; continued reheating gives rise to toxicity.
- Due to nutritional variation, some strains may grow poorly or fail to grow on this medium.
- Some glucose-fermenting organisms which are able to reduce the pH of the medium to a critical level may not survive in this medium. Early sub-culture is necessary to isolate these organisms.
Presentation
| Presentation | 20 tubes, 16 x 113 mm |
|---|---|
| Filling | 9 ± 0.1 mL |
| Packaging | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metal non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 15.000 |
| Yeast extract | 5.000 |
| D(+) Glucose | 5.500 |
| Sodium chloride | 2.500 |
| Sodium thioglycolate | 0.500 |
| L-Cystine | 0.500 |
| Resazurin | 0.001 |
| Agar | 0.750 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.1 ± 0.2 at 25 °C |
Microbiological control
Inoculate: 50-100 CFU (productivity) according to harmonized Eur. Pharmacopoeia and ISO 11133 standard.
Aerobic. Incubation at 30-35 °C for 18-72 h (bacteria) and 20-25 °C for 3-5 days (moulds and yeast).
Cl. perfringens ATCC 13124 at 37 °C, reading after 24 ± 3 h.
| Microorganism | Growth |
|---|---|
| Clostridium sporogenesATCC 19404, WDCM 00008 | Good - in anaerobic zone |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good - in aerobic zone |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good - in aerobic and anaerobic zone |
| Candida albicansATCC 10231, WDCM 00054 | Good - in aerobic zone |
| Aspergillus brasiliensisATCC 16404 | Good - in aerobic zone |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good - in aerobic zone |
| Clostridium perfringensATCC 13124, WDCM 00007 (37 °C) | Good - in anaerobic zone |
Sterility control
Incubation 14 days at 22.5 ± 2 °C: NO GROWTH.
Incubation 14 days at 32.5 ± 2 °C: NO GROWTH.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press Inc. London.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Food. 3rd ed. A.P.H.A. Washington. DC.
- EUROPEAN PHARMACOPOEIA 7.0 (2011) 7th ed. § 2.6.1. Sterility. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- FDA (Food and Drug Administration) (1998) Bacteriological Analytical Manual, 8th ed. Rev. A. AOAC International. Gaithersburg. MD.
- HORWITZ, W. (2000) Official Methods of Analysis. 17th ed. AOAC International. Gaithersburg. MD.
- ISENBERG, H.D. (Ed.) (1998) Essential Procedures for Clinical Microbiology. ASM. Washington. USA.
- ISO 7937 (2004) Microbiology of Food and Animal Feeding Stuffs. Horizontal Method for Enumeration of C. perfringens. Colony-count technique.
- MacFADDIN, J.F. (1985) Media for Isolation-cultivation-identification-maintenance of medical bacteria. Vol. I. Williams & Wilkins. Baltimore. MD. USA.
- USP 33 - NF 28 (2011) <71> Sterility Tests. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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