Extraction and purification
PureIT ExoZAP PCR CleanUp
One-step enzymatic clean-up of PCR products, with no columns or beads
- Cat. No.
- A620601
- Category
- Extraction and purification
- Formats
- 4 formats
- Manufacturer
A balanced mix of heat-labile exonuclease I and recombinant shrimp alkaline phosphatase for cleaning up PCR products in a single step. The exonuclease degrades leftover primers and single-stranded DNA while the phosphatase dephosphorylates excess dNTPs; the reagent is added straight to the reaction, works in common PCR buffers and is then fully heat-inactivated. No spin columns or magnetic beads are needed and there is no sample loss.
Key features
5-minute protocol
Incubation at 37 °C and inactivation at 80 °C, in the same PCR tube.
No sample loss
The PCR product stays in the tube, with no binding or elution steps.
No columns or beads
Simply add the reagent to the PCR product.
Complete inactivation
Heating to 80 °C inactivates both enzymes so they do not affect downstream work.
Applications
- Sanger sequencing
- Primer extension reactions
- SNP analysis
- NGS library preparation
Technical details
| Composition | Heat-labile exonuclease I (HL-ExoI) and recombinant shrimp alkaline phosphatase (rSAP) |
|---|---|
| Dosage | 2 µL per 5 µL of PCR product; scale up proportionally for larger volumes |
| Incubation | 37 °C for 2-5 minutes |
| Inactivation | 80 °C for 3-10 minutes |
| Storage | -20 °C; keep on ice during use |
| Shipping | On dry ice |
| Quality control | Functional test by Sanger sequencing of PCR product spiked with dNTPs and primers; enzymes tested for double- and single-stranded endonuclease activity |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.