DNA polymerases
Taq DNA Polymerase Glycerol Free, 50 U/µL
Concentrated glycerol-free Taq DNA polymerase (50 U/µL) for lyophilisation, automation and kit manufacturing
- Cat. No.
- A490010
- Category
- DNA polymerases
- Formats
- 3 formats
- Manufacturer
Taq DNA polymerase in a glycerol-free storage buffer at a high concentration of 50 U/µL. Without glycerol it is well suited to lyophilisation, automated high-throughput workflows and any setup where small volumes must be pipetted accurately; the concentrated format is intended for bulk master mix production, robotic pipetting and lyophilised mixes for kits. It has 5'-3' polymerase and 5'-3' exonuclease activity, no proofreading activity, and leaves a 3' A overhang that is convenient for TA cloning.
Key features
Glycerol free
Glycerol-free storage buffer, ideal for lyophilisation and automated dispensing.
High concentration
50 U/µL to formulate concentrated master mixes with minimal enzyme volumes.
High stability
Retains about 90% of its activity after 50 freeze-thaw cycles.
Amplicons up to 5 kb
Also incorporates dUTP and leaves a 3' A overhang for TA cloning.
Applications
- Lyophilisation of PCR master mixes
- Automated high-throughput testing
- Robotic pipetting
- Bulk kit and master mix manufacturing
- DNA target detection by PCR
- TA cloning and primer extension
Technical details
| Concentration | 50 U/µL |
|---|---|
| Storage buffer | 20 mM Tris-HCl pH 8.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% polysorbate 20, glycerol free |
| Activity | 5'-3' polymerase and 5'-3' exonuclease; no 3'-5' exonuclease activity |
| Product ends | 3' A overhang |
| Amplicon length | Up to 5 kb |
| Unit definition | 1 unit incorporates 10 nmol of dNTPs into acid-precipitable DNA in 30 minutes at 72 °C |
| Lyophilisation | Requires cryoprotectants such as mannitol or trehalose; formulation to be optimised |
| Supplied with | Enzyme only, no reaction buffer |
| Storage | -20 °C long term; 4 °C for up to 6 months |
| Shipping | On dry ice |
| Quality control | No contaminating endonuclease, nicking or exonuclease activities |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
DNA polymerasesSuperSens Taq DNA Polymerase GreenCat. No. DB-1329Formats 3 formatsFrequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.