DNA polymerases
GC-rich DNA Target Kit
Hot start kit with two dedicated buffers to amplify and optimise GC-rich DNA targets
- Cat. No.
- A227103
- Category
- DNA polymerases
- Formats
- 1 format
- Manufacturer
The GC-rich DNA Target Kit is designed for amplifying GC-rich DNA sequences and for finding the best conditions before moving to the ready-to-use GC TEMPase master mixes. It supplies TEMPase Hot Start DNA Polymerase (5 U/µL), the two 4x buffers GC Buffer I and GC Buffer II, and 25 mM MgCl2 in separate tubes, so both buffers can be compared and magnesium adjusted. The polymerase is a chemically modified Taq that stays inactive at room temperature and is activated by an initial 15-minute step at 95 °C.
Key features
Two GC-rich buffers
GC Buffer I and GC Buffer II allow two optimised conditions for high-GC targets to be tested side by side.
Chemical hot start
A chemical group blocks the active site until heat activation, preventing extension of mis-primed primers during setup.
Room temperature setup
Reactions can be assembled without ice for convenient handling.
High yield and specificity
High sensitivity and yield with reduced formation of non-specific products.
Applications
- Amplification of GC-rich DNA targets
- Optimisation of PCR assays that fail with standard buffers
- Buffer selection before moving to GC TEMPase master mixes
- PCR from genomic, plasmid and bacterial DNA
Technical details
| Polymerase | TEMPase Hot Start DNA Polymerase, 5 U/µL (chemically modified Taq) |
|---|---|
| Composition | 100 µL enzyme, 1.5 mL 4x GC Buffer I, 1.5 mL 4x GC Buffer II, 1.5 mL 25 mM MgCl2 |
| MgCl2 in GC buffers | 6 mM in the 4x buffer, 1.5 mM in the reaction |
| Activation | 15 minutes at 95 °C |
| Extension | About 1,000 bases per minute at 72 °C |
| Enzyme storage buffer | 20 mM Tris-HCl pH 8.9, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% polysorbate 20, 50% glycerol |
| Unit definition | 1 unit incorporates 10 nmol of dNTPs into acid-precipitable DNA in 30 minutes at 72 °C |
| Storage | -20 °C long term; 4 °C for up to 6 months |
| Shipping | On dry ice |
| Quality control | Enzyme tested for absence of contaminating endonuclease, nicking and exonuclease activities |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
DNA polymerasesSuperSens Taq DNA Polymerase GreenCat. No. DB-1329Formats 3 formatsFrequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.